column purification Search Results


pcr purification kit  (Jena Bioscience)
94
Jena Bioscience pcr purification kit
Pcr Purification Kit, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pcr purification kit/product/Jena Bioscience
Average 94 stars, based on 1 article reviews
pcr purification kit - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
his60 ni gravity column  (TaKaRa)
94
TaKaRa his60 ni gravity column
His60 Ni Gravity Column, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/his60 ni gravity column/product/TaKaRa
Average 94 stars, based on 1 article reviews
his60 ni gravity column - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
rnasep prep rna purification column  (ADS Biotec)
93
ADS Biotec rnasep prep rna purification column
Methylation of the first transcribed nucleotides in mRNA only partially prevents protein level decrease under IFN-induced stress. Relative protein production levels 72 h after a 5 h IFNα pre-treatment in ( A and B ) A549 and ( C and D ) JAWS II cells. Data were analysed for three independent replicates (each experiment consisted of three technical replicates). Bars for each transcript represent the mean value ± SEM normalized to protein production in mock-treated cells. Statistical significance: * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001 (one-way ANOVA with Turkey's multiple comparisons test) (data presenting changes in relative protein production with increasing concentration of IFNα are shown in for A549 and JAWS II cells, respectively). <t>Co-purification</t> of endogenous proteins from lysates of IFNα-treated ( E and F ) A549 (IFNα concentration was 500 U/ml) and ( G and H ) JAWS II (IFNα concentration was 5000 U/ml) cells with biotinylated <t>RNA</t> bearing cap0, cap1, cap2 or cap2-1 with ( E and G ) A or ( F and H ) m 6 A as the first transcribed nucleotide. Human eIF4E, IFIT1 and NCBP1 or murine counterparts (Ifit1b: lower band) were detected in precipitates by western blotting. A 1/15th and 1/21st volume of lysate used for each incubation with beads was loaded as input sample for ( E–G ) and ( H ), respectively; a 1/3rd volume of each eluate was loaded; shorter exposure is presented for input samples than for eluates; *non-specific band.
Rnasep Prep Rna Purification Column, supplied by ADS Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnasep prep rna purification column/product/ADS Biotec
Average 93 stars, based on 1 article reviews
rnasep prep rna purification column - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
spin easy dna pro kit for soil  (Valiant Co Ltd)
94
Valiant Co Ltd spin easy dna pro kit for soil
Methylation of the first transcribed nucleotides in mRNA only partially prevents protein level decrease under IFN-induced stress. Relative protein production levels 72 h after a 5 h IFNα pre-treatment in ( A and B ) A549 and ( C and D ) JAWS II cells. Data were analysed for three independent replicates (each experiment consisted of three technical replicates). Bars for each transcript represent the mean value ± SEM normalized to protein production in mock-treated cells. Statistical significance: * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001 (one-way ANOVA with Turkey's multiple comparisons test) (data presenting changes in relative protein production with increasing concentration of IFNα are shown in for A549 and JAWS II cells, respectively). <t>Co-purification</t> of endogenous proteins from lysates of IFNα-treated ( E and F ) A549 (IFNα concentration was 500 U/ml) and ( G and H ) JAWS II (IFNα concentration was 5000 U/ml) cells with biotinylated <t>RNA</t> bearing cap0, cap1, cap2 or cap2-1 with ( E and G ) A or ( F and H ) m 6 A as the first transcribed nucleotide. Human eIF4E, IFIT1 and NCBP1 or murine counterparts (Ifit1b: lower band) were detected in precipitates by western blotting. A 1/15th and 1/21st volume of lysate used for each incubation with beads was loaded as input sample for ( E–G ) and ( H ), respectively; a 1/3rd volume of each eluate was loaded; shorter exposure is presented for input samples than for eluates; *non-specific band.
Spin Easy Dna Pro Kit For Soil, supplied by Valiant Co Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/spin easy dna pro kit for soil/product/Valiant Co Ltd
Average 94 stars, based on 1 article reviews
spin easy dna pro kit for soil - by Bioz Stars, 2026-02
94/100 stars
  Buy from Supplier
total rna  (Jena Bioscience)
96
Jena Bioscience total rna
Methylation of the first transcribed nucleotides in mRNA only partially prevents protein level decrease under IFN-induced stress. Relative protein production levels 72 h after a 5 h IFNα pre-treatment in ( A and B ) A549 and ( C and D ) JAWS II cells. Data were analysed for three independent replicates (each experiment consisted of three technical replicates). Bars for each transcript represent the mean value ± SEM normalized to protein production in mock-treated cells. Statistical significance: * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001 (one-way ANOVA with Turkey's multiple comparisons test) (data presenting changes in relative protein production with increasing concentration of IFNα are shown in for A549 and JAWS II cells, respectively). <t>Co-purification</t> of endogenous proteins from lysates of IFNα-treated ( E and F ) A549 (IFNα concentration was 500 U/ml) and ( G and H ) JAWS II (IFNα concentration was 5000 U/ml) cells with biotinylated <t>RNA</t> bearing cap0, cap1, cap2 or cap2-1 with ( E and G ) A or ( F and H ) m 6 A as the first transcribed nucleotide. Human eIF4E, IFIT1 and NCBP1 or murine counterparts (Ifit1b: lower band) were detected in precipitates by western blotting. A 1/15th and 1/21st volume of lysate used for each incubation with beads was loaded as input sample for ( E–G ) and ( H ), respectively; a 1/3rd volume of each eluate was loaded; shorter exposure is presented for input samples than for eluates; *non-specific band.
Total Rna, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/total rna/product/Jena Bioscience
Average 96 stars, based on 1 article reviews
total rna - by Bioz Stars, 2026-02
96/100 stars
  Buy from Supplier
dna purification buffers  (Cell Signaling Technology Inc)
97
Cell Signaling Technology Inc dna purification buffers
Methylation of the first transcribed nucleotides in mRNA only partially prevents protein level decrease under IFN-induced stress. Relative protein production levels 72 h after a 5 h IFNα pre-treatment in ( A and B ) A549 and ( C and D ) JAWS II cells. Data were analysed for three independent replicates (each experiment consisted of three technical replicates). Bars for each transcript represent the mean value ± SEM normalized to protein production in mock-treated cells. Statistical significance: * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001 (one-way ANOVA with Turkey's multiple comparisons test) (data presenting changes in relative protein production with increasing concentration of IFNα are shown in for A549 and JAWS II cells, respectively). <t>Co-purification</t> of endogenous proteins from lysates of IFNα-treated ( E and F ) A549 (IFNα concentration was 500 U/ml) and ( G and H ) JAWS II (IFNα concentration was 5000 U/ml) cells with biotinylated <t>RNA</t> bearing cap0, cap1, cap2 or cap2-1 with ( E and G ) A or ( F and H ) m 6 A as the first transcribed nucleotide. Human eIF4E, IFIT1 and NCBP1 or murine counterparts (Ifit1b: lower band) were detected in precipitates by western blotting. A 1/15th and 1/21st volume of lysate used for each incubation with beads was loaded as input sample for ( E–G ) and ( H ), respectively; a 1/3rd volume of each eluate was loaded; shorter exposure is presented for input samples than for eluates; *non-specific band.
Dna Purification Buffers, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/dna purification buffers/product/Cell Signaling Technology Inc
Average 97 stars, based on 1 article reviews
dna purification buffers - by Bioz Stars, 2026-02
97/100 stars
  Buy from Supplier
monarch rna purification columns  (New England Biolabs)
99
New England Biolabs monarch rna purification columns
Methylation of the first transcribed nucleotides in mRNA only partially prevents protein level decrease under IFN-induced stress. Relative protein production levels 72 h after a 5 h IFNα pre-treatment in ( A and B ) A549 and ( C and D ) JAWS II cells. Data were analysed for three independent replicates (each experiment consisted of three technical replicates). Bars for each transcript represent the mean value ± SEM normalized to protein production in mock-treated cells. Statistical significance: * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001 (one-way ANOVA with Turkey's multiple comparisons test) (data presenting changes in relative protein production with increasing concentration of IFNα are shown in for A549 and JAWS II cells, respectively). <t>Co-purification</t> of endogenous proteins from lysates of IFNα-treated ( E and F ) A549 (IFNα concentration was 500 U/ml) and ( G and H ) JAWS II (IFNα concentration was 5000 U/ml) cells with biotinylated <t>RNA</t> bearing cap0, cap1, cap2 or cap2-1 with ( E and G ) A or ( F and H ) m 6 A as the first transcribed nucleotide. Human eIF4E, IFIT1 and NCBP1 or murine counterparts (Ifit1b: lower band) were detected in precipitates by western blotting. A 1/15th and 1/21st volume of lysate used for each incubation with beads was loaded as input sample for ( E–G ) and ( H ), respectively; a 1/3rd volume of each eluate was loaded; shorter exposure is presented for input samples than for eluates; *non-specific band.
Monarch Rna Purification Columns, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/monarch rna purification columns/product/New England Biolabs
Average 99 stars, based on 1 article reviews
monarch rna purification columns - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
column purification  (New England Biolabs)
99
New England Biolabs column purification
Methylation of the first transcribed nucleotides in mRNA only partially prevents protein level decrease under IFN-induced stress. Relative protein production levels 72 h after a 5 h IFNα pre-treatment in ( A and B ) A549 and ( C and D ) JAWS II cells. Data were analysed for three independent replicates (each experiment consisted of three technical replicates). Bars for each transcript represent the mean value ± SEM normalized to protein production in mock-treated cells. Statistical significance: * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001 (one-way ANOVA with Turkey's multiple comparisons test) (data presenting changes in relative protein production with increasing concentration of IFNα are shown in for A549 and JAWS II cells, respectively). <t>Co-purification</t> of endogenous proteins from lysates of IFNα-treated ( E and F ) A549 (IFNα concentration was 500 U/ml) and ( G and H ) JAWS II (IFNα concentration was 5000 U/ml) cells with biotinylated <t>RNA</t> bearing cap0, cap1, cap2 or cap2-1 with ( E and G ) A or ( F and H ) m 6 A as the first transcribed nucleotide. Human eIF4E, IFIT1 and NCBP1 or murine counterparts (Ifit1b: lower band) were detected in precipitates by western blotting. A 1/15th and 1/21st volume of lysate used for each incubation with beads was loaded as input sample for ( E–G ) and ( H ), respectively; a 1/3rd volume of each eluate was loaded; shorter exposure is presented for input samples than for eluates; *non-specific band.
Column Purification, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/column purification/product/New England Biolabs
Average 99 stars, based on 1 article reviews
column purification - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier
rnasep semipreparative column  (ADS Biotec)
93
ADS Biotec rnasep semipreparative column
Methylation of the first transcribed nucleotides in mRNA only partially prevents protein level decrease under IFN-induced stress. Relative protein production levels 72 h after a 5 h IFNα pre-treatment in ( A and B ) A549 and ( C and D ) JAWS II cells. Data were analysed for three independent replicates (each experiment consisted of three technical replicates). Bars for each transcript represent the mean value ± SEM normalized to protein production in mock-treated cells. Statistical significance: * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001 (one-way ANOVA with Turkey's multiple comparisons test) (data presenting changes in relative protein production with increasing concentration of IFNα are shown in for A549 and JAWS II cells, respectively). <t>Co-purification</t> of endogenous proteins from lysates of IFNα-treated ( E and F ) A549 (IFNα concentration was 500 U/ml) and ( G and H ) JAWS II (IFNα concentration was 5000 U/ml) cells with biotinylated <t>RNA</t> bearing cap0, cap1, cap2 or cap2-1 with ( E and G ) A or ( F and H ) m 6 A as the first transcribed nucleotide. Human eIF4E, IFIT1 and NCBP1 or murine counterparts (Ifit1b: lower band) were detected in precipitates by western blotting. A 1/15th and 1/21st volume of lysate used for each incubation with beads was loaded as input sample for ( E–G ) and ( H ), respectively; a 1/3rd volume of each eluate was loaded; shorter exposure is presented for input samples than for eluates; *non-specific band.
Rnasep Semipreparative Column, supplied by ADS Biotec, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rnasep semipreparative column/product/ADS Biotec
Average 93 stars, based on 1 article reviews
rnasep semipreparative column - by Bioz Stars, 2026-02
93/100 stars
  Buy from Supplier
histalon gravity column purification kit  (TaKaRa)
95
TaKaRa histalon gravity column purification kit
Methylation of the first transcribed nucleotides in mRNA only partially prevents protein level decrease under IFN-induced stress. Relative protein production levels 72 h after a 5 h IFNα pre-treatment in ( A and B ) A549 and ( C and D ) JAWS II cells. Data were analysed for three independent replicates (each experiment consisted of three technical replicates). Bars for each transcript represent the mean value ± SEM normalized to protein production in mock-treated cells. Statistical significance: * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001 (one-way ANOVA with Turkey's multiple comparisons test) (data presenting changes in relative protein production with increasing concentration of IFNα are shown in for A549 and JAWS II cells, respectively). <t>Co-purification</t> of endogenous proteins from lysates of IFNα-treated ( E and F ) A549 (IFNα concentration was 500 U/ml) and ( G and H ) JAWS II (IFNα concentration was 5000 U/ml) cells with biotinylated <t>RNA</t> bearing cap0, cap1, cap2 or cap2-1 with ( E and G ) A or ( F and H ) m 6 A as the first transcribed nucleotide. Human eIF4E, IFIT1 and NCBP1 or murine counterparts (Ifit1b: lower band) were detected in precipitates by western blotting. A 1/15th and 1/21st volume of lysate used for each incubation with beads was loaded as input sample for ( E–G ) and ( H ), respectively; a 1/3rd volume of each eluate was loaded; shorter exposure is presented for input samples than for eluates; *non-specific band.
Histalon Gravity Column Purification Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/histalon gravity column purification kit/product/TaKaRa
Average 95 stars, based on 1 article reviews
histalon gravity column purification kit - by Bioz Stars, 2026-02
95/100 stars
  Buy from Supplier
purification maxiprep columns  (TaKaRa)
99
TaKaRa purification maxiprep columns
Methylation of the first transcribed nucleotides in mRNA only partially prevents protein level decrease under IFN-induced stress. Relative protein production levels 72 h after a 5 h IFNα pre-treatment in ( A and B ) A549 and ( C and D ) JAWS II cells. Data were analysed for three independent replicates (each experiment consisted of three technical replicates). Bars for each transcript represent the mean value ± SEM normalized to protein production in mock-treated cells. Statistical significance: * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001 (one-way ANOVA with Turkey's multiple comparisons test) (data presenting changes in relative protein production with increasing concentration of IFNα are shown in for A549 and JAWS II cells, respectively). <t>Co-purification</t> of endogenous proteins from lysates of IFNα-treated ( E and F ) A549 (IFNα concentration was 500 U/ml) and ( G and H ) JAWS II (IFNα concentration was 5000 U/ml) cells with biotinylated <t>RNA</t> bearing cap0, cap1, cap2 or cap2-1 with ( E and G ) A or ( F and H ) m 6 A as the first transcribed nucleotide. Human eIF4E, IFIT1 and NCBP1 or murine counterparts (Ifit1b: lower band) were detected in precipitates by western blotting. A 1/15th and 1/21st volume of lysate used for each incubation with beads was loaded as input sample for ( E–G ) and ( H ), respectively; a 1/3rd volume of each eluate was loaded; shorter exposure is presented for input samples than for eluates; *non-specific band.
Purification Maxiprep Columns, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/purification maxiprep columns/product/TaKaRa
Average 99 stars, based on 1 article reviews
purification maxiprep columns - by Bioz Stars, 2026-02
99/100 stars
  Buy from Supplier

Image Search Results


Methylation of the first transcribed nucleotides in mRNA only partially prevents protein level decrease under IFN-induced stress. Relative protein production levels 72 h after a 5 h IFNα pre-treatment in ( A and B ) A549 and ( C and D ) JAWS II cells. Data were analysed for three independent replicates (each experiment consisted of three technical replicates). Bars for each transcript represent the mean value ± SEM normalized to protein production in mock-treated cells. Statistical significance: * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001 (one-way ANOVA with Turkey's multiple comparisons test) (data presenting changes in relative protein production with increasing concentration of IFNα are shown in for A549 and JAWS II cells, respectively). Co-purification of endogenous proteins from lysates of IFNα-treated ( E and F ) A549 (IFNα concentration was 500 U/ml) and ( G and H ) JAWS II (IFNα concentration was 5000 U/ml) cells with biotinylated RNA bearing cap0, cap1, cap2 or cap2-1 with ( E and G ) A or ( F and H ) m 6 A as the first transcribed nucleotide. Human eIF4E, IFIT1 and NCBP1 or murine counterparts (Ifit1b: lower band) were detected in precipitates by western blotting. A 1/15th and 1/21st volume of lysate used for each incubation with beads was loaded as input sample for ( E–G ) and ( H ), respectively; a 1/3rd volume of each eluate was loaded; shorter exposure is presented for input samples than for eluates; *non-specific band.

Journal: Nucleic Acids Research

Article Title: 2′- O -Methylation of the second transcribed nucleotide within the mRNA 5′ cap impacts the protein production level in a cell-specific manner and contributes to RNA immune evasion

doi: 10.1093/nar/gkac722

Figure Lengend Snippet: Methylation of the first transcribed nucleotides in mRNA only partially prevents protein level decrease under IFN-induced stress. Relative protein production levels 72 h after a 5 h IFNα pre-treatment in ( A and B ) A549 and ( C and D ) JAWS II cells. Data were analysed for three independent replicates (each experiment consisted of three technical replicates). Bars for each transcript represent the mean value ± SEM normalized to protein production in mock-treated cells. Statistical significance: * P <0.05, ** P <0.01, *** P <0.001, **** P <0.0001 (one-way ANOVA with Turkey's multiple comparisons test) (data presenting changes in relative protein production with increasing concentration of IFNα are shown in for A549 and JAWS II cells, respectively). Co-purification of endogenous proteins from lysates of IFNα-treated ( E and F ) A549 (IFNα concentration was 500 U/ml) and ( G and H ) JAWS II (IFNα concentration was 5000 U/ml) cells with biotinylated RNA bearing cap0, cap1, cap2 or cap2-1 with ( E and G ) A or ( F and H ) m 6 A as the first transcribed nucleotide. Human eIF4E, IFIT1 and NCBP1 or murine counterparts (Ifit1b: lower band) were detected in precipitates by western blotting. A 1/15th and 1/21st volume of lysate used for each incubation with beads was loaded as input sample for ( E–G ) and ( H ), respectively; a 1/3rd volume of each eluate was loaded; shorter exposure is presented for input samples than for eluates; *non-specific band.

Article Snippet: The crude mRNAs were purified using NucleoSpin RNA Clean-up XS (740903, Macherey-Nagel). mRNAs were HPLC-purified using an RNASep Prep RNA Purification Column (ADS Biotec) at 55°C, a linear gradient of buffer B (0.1 M triethylammonium acetate pH 7.0 and 50% acetonitrile) from 17.5% to 25.8% in buffer A (0.1 M triethylammonium acetate pH 7.0) over 20 min and a flow rate of 0.9 ml/min. mRNAs from collected fractions were recovered by isopropanol precipitation.

Techniques: Methylation, Concentration Assay, Copurification, Western Blot, Incubation